IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/19: Difference between revisions
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(Autocreate 2008/08/19 Entry for IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation) |
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== | ==Results of AgrA and C transformation== | ||
Transformation has failed. No colonies were visible for the plates of Agr A or C. The Puc9 positive control grew. | |||
We believe that the problem was in the gel-extraction between ligation and transformation. Most of our plasmid was probably lost in this step. Next time we will directly use the results from the ligation reaction to transform. Although many bands were seen on the previous gel of the ligation reaction, we will check our transformation growth by single colony PCR to confirm transformation of the plasmid with our correct insert. | |||
Revision as of 10:41, 19 August 2008
 Turing Pattern Formation
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Results of AgrA and C transformationTransformation has failed. No colonies were visible for the plates of Agr A or C. The Puc9 positive control grew. We believe that the problem was in the gel-extraction between ligation and transformation. Most of our plasmid was probably lost in this step. Next time we will directly use the results from the ligation reaction to transform. Although many bands were seen on the previous gel of the ligation reaction, we will check our transformation growth by single colony PCR to confirm transformation of the plasmid with our correct insert.
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