IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/20: Difference between revisions
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- '''Result''' : 2 bands (a little bit more than 3000b and a little bit more than 5000b) :ok! | - '''Result''' : 2 bands (a little bit more than 3000b and a little bit more than 5000b) :ok! | ||
==Ligation== | |||
- Materials : | |||
*AgrA | |||
*AgrB | |||
*AgrC | |||
*AgrD | |||
*Pupp | |||
*Pspac | |||
*Ppac | |||
*Pxyl | |||
- Double digest of PCR products | |||
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Revision as of 04:04, 21 August 2008
Turing Pattern Formation | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Check Lux components- Single colony PCR for :
- Protocol : add 1μL of cells (diluted in water), 10μL of Master Mis, 7μL of SDW, 1μL of VF primer and 1μL of VR primer - Gel PCR products - Results
Check vector ECE190- Plasmid miniprep - Nanodrop - Double digest : 6μL of SDW, 8μL of DNA, 1μL of buffer, 1μL of XbaI and 1μL of PstI - Run on a gel - Result : 2 bands (a little bit more than 3000b and a little bit more than 5000b) :ok! Ligation- Materials :
- Double digest of PCR products |