IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/20: Difference between revisions
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- Gel PCR products | - Gel PCR products | ||
Gel 1 | |||
* Lane2 : Hyperladder1 | |||
* Lane3 : JO4630, colony 1 | |||
* Lane4 : JO4630, colony 2 | |||
* Lane5 : JO4630, colony 3 | |||
* Lane6 : JO4630, colony 4 | |||
* Lane7 : HyperladderI | |||
[[Image:photon.gif|300px|center]] | |||
Gel 2 | |||
* Lane2 : HyperladderI | |||
* Lane3 : ECE190 double digest | |||
* Lane4 : S0168, colony 1 | |||
* Lane5 : S0168, colony 2 | |||
* Lane6 : S0168, colony 3 | |||
* Lane7 : S0168, colony 4 | |||
* Lane8 : HyperladderI | |||
* Lane9 : R0040 | |||
* Lane10 : R0062 | |||
* Lane11 : Ladder 100bp | |||
[[Image:photoo.gif|300px|center]] | |||
- '''Results''' | - '''Results''' |
Revision as of 06:18, 5 September 2008
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Check Lux components- Single colony PCR for :
- Protocol : add 1μL of cells (diluted in water), 10μL of Master Mis, 7μL of SDW, 1μL of VF primer and 1μL of VR primer - Gel PCR products Gel 1
Gel 2
- Results
Check vector ECE190- Plasmid miniprep - Nanodrop - Double digest : 6μL of SDW, 8μL of DNA, 1μL of buffer, 1μL of XbaI and 1μL of PstI - Run on a gel - Result : 2 bands (a little bit more than 3000b and a little bit more than 5000b) :ok! Ligation- Materials :
- Double digest of PCR products - Run vector, AgrA and AgrD on a gel - DNA clean and concentrator for AgrA, B,C and D, promoters - Microclean for both RBS - Nanodrop
- Extract plasmid annd Agr from gel and clean - Ligation |