IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/20: Difference between revisions
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(Autocreate 2008/08/20 Entry for IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation) |
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== | ==Check Lux components== | ||
- Single colony PCR for : | |||
*R0040 (MIT stuff) | |||
*R0062 (MIT stuff) | |||
*4 different colonies of S0168 (from a transformation plate from 12/08) | |||
*4 different colonies of J04630 (from a transformation plate from 12/08) | |||
- Protocol : add 1μL of cells (diluted in water), 10μL of Master Mis, 7μL of SDW, 1μL of VF primer and 1μL of VR primer | |||
- Gel PCR products | |||
- '''Results''' | |||
* R0040 and R0062 : one big band of about 300b (expected size 293), OK! | |||
* S0168 : one band of about 400b for the 4 different colonies (expected size 1234!), bad! This plate does not contain S0168 | |||
* J04630 (colonies 2 and 4) : one band of about 1100b (expected size 1173), OK! | |||
* J04630 (colony 1) : one good band plus another band... | |||
* J04630 (colony 3) : one band of about 600b, bad! | |||
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Revision as of 03:57, 21 August 2008
 Turing Pattern Formation
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Check Lux components- Single colony PCR for :
- Protocol : add 1μL of cells (diluted in water), 10μL of Master Mis, 7μL of SDW, 1μL of VF primer and 1μL of VR primer - Gel PCR products - Results
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