IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/20: Difference between revisions
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*Ppac | *Ppac | ||
*Pxyl | *Pxyl | ||
*RBS S | |||
*RBS W | |||
*psB4C5 | |||
- Double digest of PCR products | - Double digest of PCR products | ||
- Run vector, AgrA and AgrD on a gel | |||
- DNA clean and concentrator for AgrA, B,C and D, promoters | |||
- Microclean for both RBS | |||
- Nanodrop | |||
- Extract plasmid annd Agr from gel and clean | |||
- Ligation | |||
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Revision as of 04:09, 21 August 2008
 Turing Pattern Formation
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Check Lux components- Single colony PCR for :
- Protocol : add 1μL of cells (diluted in water), 10μL of Master Mis, 7μL of SDW, 1μL of VF primer and 1μL of VR primer - Gel PCR products - Results
Check vector ECE190- Plasmid miniprep - Nanodrop - Double digest : 6μL of SDW, 8μL of DNA, 1μL of buffer, 1μL of XbaI and 1μL of PstI - Run on a gel - Result : 2 bands (a little bit more than 3000b and a little bit more than 5000b) :ok! Ligation- Materials :
- Double digest of PCR products - Run vector, AgrA and AgrD on a gel - DNA clean and concentrator for AgrA, B,C and D, promoters - Microclean for both RBS - Nanodrop - Extract plasmid annd Agr from gel and clean - Ligation | |
