IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/21: Difference between revisions
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(Autocreate 2008/08/21 Entry for IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation) |
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== | ==Transformation of ligation products== | ||
- Spin chemically competent TOP10, add 100μL of CaCl2 solution | |||
- Add 5μL of DNA (ligation products), and 1μL of PUC9 | |||
- Continue the protocol of transformation | |||
==J04630== | |||
- Plate good colonies from yesterday on Kan25 and put in LB (for plasmid stock for tomorrow) | |||
==Plasmid stocks== | |||
- Plasmid miniprep R0040 and R0062 | |||
Revision as of 04:20, 21 August 2008
 Turing Pattern Formation
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Transformation of ligation products- Spin chemically competent TOP10, add 100μL of CaCl2 solution - Add 5μL of DNA (ligation products), and 1μL of PUC9 - Continue the protocol of transformation J04630- Plate good colonies from yesterday on Kan25 and put in LB (for plasmid stock for tomorrow) Plasmid stocks- Plasmid miniprep R0040 and R0062
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