IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/21

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Revision as of 07:39, 5 September 2008



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Transformation of ligation products

- Spin chemically competent TOP10, add 100μL of CaCl2 solution

- Add 5μL of DNA (ligation products), and 1μL of PUC9

- Continue the protocol of transformation

J04630

- Plate good colonies from yesterday on Kan25 and put in LB (for plasmid stock for tomorrow)

Plasmid stocks

- Plasmid miniprep R0040 and R0062

Check ECE188

- Plasmid miniprep

- Double digest with XbaI and PstI

- Gel

- Result : nothing! nit enough DNA


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