IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/21: Difference between revisions
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- Double digest with XbaI and PstI | - Double digest with XbaI and PstI | ||
- Gel | - Gel (lane6) | ||
- Result : nothing! | [[Image:photop.gif|300px|center]] | ||
- Result : nothing! not enough DNA | |||
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Revision as of 06:22, 5 September 2008
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Transformation of ligation products- Spin chemically competent TOP10, add 100μL of CaCl2 solution - Add 5μL of DNA (ligation products), and 1μL of PUC9 - Continue the protocol of transformation J04630- Plate good colonies from yesterday on Kan25 and put in LB (for plasmid stock for tomorrow) Plasmid stocks- Plasmid miniprep R0040 and R0062 Check ECE188- Plasmid miniprep - Double digest with XbaI and PstI - Gel (lane6)  - Result : nothing! not enough DNA | |
