IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/22: Difference between revisions

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|style="background-color: #EEE"|[[Image:IgemCam.jpg|200px]]<span style="font-size:22px;"> Turing Pattern Formation</span>
|style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]]
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==Stock ECE112, 166, 153==
- Plasmid miniprep (2 tubes of ECE112, 2 tubes of ECE166 and 1 tube of ECE153)
- Nanodrop
==Transformation of Bacillus==
* For strain IA751 : transform with ECE166, 171, 153 and 112
* For strain IA771 : transform with ECE166, 171
* for glycerol stocks (one with 100μL of medium B and glycerol, and one with only glycerol) : transform with ECE166
- We used the same protocol than before with a few changes :
* add sterile 50% glucose in medium A
* don't keep samples after using them for spectrophotometer
* spin each tubes and keep only 100μL of medium A (to concentrate cells) before adding DNA




==New PCR of promoters==
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Revision as of 08:57, 5 September 2008

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New PCR of promoters


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