IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/22: Difference between revisions

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==Stock ECE112, 166, 153==


- Plasmid miniprep (2 tubes of ECE112, 2 tubes of ECE166 and 1 tube of ECE153)
- Nanodrop
==Transformation of Bacillus==
* For strain IA751 : transform with ECE166, 171, 153 and 112
* For strain IA771 : transform with ECE166, 171
* for glycerol stocks (one with 100μL of medium B and glycerol, and one with only glycerol) : transform with ECE166
- We used the same protocol than before with a few changes :
* add sterile 50% glucose in medium A
* don't keep samples after using them for spectrophotometer
* spin each tubes and keep only 100μL of medium A (to concentrate cells) before adding DNA
{|class="wikitable" style="text-align:center" border="1"
|-
! Time (min) !! 0 !! 20 !! 40 !! 60 !! 80 !! 100 !! 120 !! 130 !! 140
|-
| OD650 for IA751 || 0.1817 || 0.1870 || 0.1929 || 0.2115 || 0.2543 || 0.3291 || 0.3695 || 0.4188 ||0.4224
|-
| OD650 for IA751 ||0.1818 || 0.1874 || 0.1907 || 0.2088 || 0.2452 || 0.3182 || 0.3575 || 0.4228 || 0.4314
|-
|}
[[Image:graph.gif|500px|center]]
- For glycerol stocks, resuspend cells into 100μL of medium B, add DNA and wait for 30min, end plate them


==New PCR of promoters==
==New PCR of promoters==

Revision as of 08:57, 5 September 2008

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New PCR of promoters


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