IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/23: Difference between revisions

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==Results of transformation from 23/08/2008==


{|class="wikitable" style="text-align:center" border="1"
|-
!  Vector !! Strain !! Antibiotic !! number of colonies !! Conclusion
|-
| ECE166 || IA771, glycerol stock with medium B || Cm5 || one large colony and a lot of very small colonies || Contamination?
|-
| Control || IA771, glycerol stock with medium B || Cm5 || a lot of very small colonies || Contamination?
|-
| ECE166 || IA771, glycerol stock without medium B || Cm5 || many very small colonies || Contamination?
|-
| Control || IA771, glycerol stock without medium B || Cm5 || a few tiny colonies || Contamination?
|-
| ECE166 || IA751, fresh || Cm5 || 9 large colonies, no small ones || to test
|-
| Control || IA751, fresh || Cm5 || no growth, scratches || ok
|-
| ECE166 || IA771, fresh || Cm5 || 1 large colony, many small worm-like growth, scratches? || to test
|-
| Control || IA771, fresh || Cm5 || no colonies at all || ok
|-
| ECE171 || IA771, fresh || Kan5 || no colonies || ??
|-
| Control || IA771, fresh || Cm5 || no colonies  || ok
|-
| ECE171 || IA751, fresh || Kan5 || about 30 large colonies and 100 small colonies || to test?
|-
| Control || IA751, fresh || Cm5 || 3 large colonies, about 20 small to medium colonies  || contamination?
|-
| ECE112 || IA751, fresh || Cm5 + Spc100 || no growth || ??
|-
| Control || IA751, fresh || Cm5 + Spc100 || no growth ||
|-
| ECE153 || IA751, fresh || Spc50 || about 500 medium to large colonies || to test
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| Control || IA751, fresh || Spc50 || no growth || ok
|}


==Check promoters (after PCR from bacillus vectors)==
==Check promoters (after PCR from bacillus vectors)==
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[[Image:photoeds.gif|250px|center]]
[[Image:photoeds.gif|250px|center]]
Size is ok.


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Revision as of 08:58, 5 September 2008

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Check promoters (after PCR from bacillus vectors)

- Gel

  • Lane1 : Hyperladder 5
  • Lane2 : Pxyl
  • Lane3 : Pspac
  • lane4 : Ppac
  • Lane5 : Pupp

Size is ok.


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