IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/23: Difference between revisions

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==Check promoters (after PCR from bacillus vectors)==
- Gel
* Lane1 : Hyperladder 5
* Lane2 : Pxyl
* Lane3 : Pspac
* lane4 : Ppac
* Lane5 : Pupp




Revision as of 08:01, 5 September 2008

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Results of transformation from 23/08/2008

Vector Strain Antibiotic number of colonies Conclusion
ECE166 IA771, glycerol stock with medium B Cm5 one large colony and a lot of very small colonies Contamination?
Control IA771, glycerol stock with medium B Cm5 a lot of very small colonies Contamination?
ECE166 IA771, glycerol stock without medium B Cm5 many very small colonies Contamination?
Control IA771, glycerol stock without medium B Cm5 a few tiny colonies Contamination?
ECE166 IA751, fresh Cm5 9 large colonies, no small ones to test
Control IA751, fresh Cm5 no growth, scratches ok
ECE166 IA771, fresh Cm5 1 large colony, many small worm-like growth, scratches? to test
Control IA771, fresh Cm5 no colonies at all ok
ECE171 IA771, fresh Kan5 no colonies ??
Control IA771, fresh Cm5 no colonies ok
ECE171 IA751, fresh Kan5 about 30 large colonies and 100 small colonies to test?
Control IA751, fresh Cm5 3 large colonies, about 20 small to medium colonies contamination?
ECE112 IA751, fresh Cm5 + Spc100 no growth ??
Control IA751, fresh Cm5 + Spc100 no growth
ECE153 IA751, fresh Spc50 about 500 medium to large colonies to test
Control IA751, fresh Spc50 no growth ok

Check promoters (after PCR from bacillus vectors)

- Gel

  • Lane1 : Hyperladder 5
  • Lane2 : Pxyl
  • Lane3 : Pspac
  • lane4 : Ppac
  • Lane5 : Pupp



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