IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/27: Difference between revisions
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- Result | - Result | ||
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! Strain !! added vector !! Observation !! Conclusion | |||
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| IA771 || none || no clearance zones || ok | |||
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| IA751 || none || big clearance zones || ok | |||
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| IA751 || ECE153 || no clearance zones || trnsformation worked! | |||
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| IA751 or IA771 || none or ECE171 || big clearance zones || we have some colonues on control plate with IA751, contamination? or antibiotic resistance? | |||
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Revision as of 01:45, 28 August 2008
 Turing Pattern Formation
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New transformation of ligation products
- Competent top 10 from the freezer - Add 5μL of ligation products, and 1.2μL of PUC9 Xylose test
- Add 1mL of culture from yesterday, 1mL of xylose (0.1g/mL) and 8mL of LB and 45μL of Spc (do that for the 4 different colonies) - Incubate 5hours - Observation with microscope : some green task, but they do not really match with bacillus cells, not conclusive! Starch plate- Add 5mL of iodine solution on starch plate, wait for 1min, throw the liquid - Result
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