IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/27
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 Turing Pattern Formation
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New transformation of ligation products
- Competent top 10 from the freezer - Add 5μL of ligation products, and 1.2μL of PUC9 Xylose test
- Add 1mL of culture from yesterday, 1mL of xylose (0.1g/mL) and 8mL of LB and 45μL of Spc (do that for the 4 different colonies) - Incubate 5hours - Observation with microscope : some green task, but they do not really match with bacillus cells, not conclusive! Starch plate- Add 5mL of iodine solution on starch plate, wait for 1min, throw the liquid - Result
Ery test- IA771 alive and IA751 dead, confirmation that we did not mix plates (same conclusion than with starch plate, last line) Test glycerol stocks of competent bacillus- Pellet bacillus cells, add 100μL of prewarmed mediumB, add 0.6μg of DNA, incubate 30min at 37°C by shaking, plate
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