IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/28: Difference between revisions

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{|{{table}} width="800"
{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:IgemCam.jpg|200px]]<span style="font-size:22px;"> Turing Pattern Formation</span>
|style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]]
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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- very short ligation products
- very short ligation products


==Results from Bacillus transformation with glycerol stocks==
==Transformation of ligation products (new)==


{|class="wikitable" style="text-align:center" border="1"
* Products to ligate : Pupp, Pspac, agrD, RBS S and RBS W
|-
!  Strain !! added vector !! Antibiotic !! number of colonies !! Conclusion
|-
| IA771 || none || blank plate || confluent colonies || cells are alive
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| IA771 || none || Cm5 || 0 || no contamination
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| IA771 || ECE166 || Cm5 || 2 || transformation ok, very low efficiency
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| IA751 || none || blank plate || confluent colonies || cells are alive
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| IA751 || none || Cm5 || 0 || no contamination
|-
| IA751 || none || Spc50 || 0 || no contamination
|-
| IA751 || ECE112 || Cm5 || 0 || problem, we can not manage to transform this vector
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| IA751 || ECE153 || Spc50 || 156 || transformation ok, good efficiency
|}


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- new fresh competent TOP 10
|}


- 5μL of DNA (1.5μL of PUC9)


__NOTOC__
- 2h30 in the incubator

Revision as of 09:08, 5 September 2008

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<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Results from transformation of ligation products

- nothing on plate, even on control plate

  • Reasons ?

- cells non competent anymore (try with fresh competent cells)

- not enough DNA (try with 10μL of DNA)

- very short ligation products

Transformation of ligation products (new)

  • Products to ligate : Pupp, Pspac, agrD, RBS S and RBS W

- new fresh competent TOP 10

- 5μL of DNA (1.5μL of PUC9)

- 2h30 in the incubator

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