IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/29: Difference between revisions
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|style="background-color: # | |style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]] | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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==Results of transformation with our ligation products== | ==Results of transformation with our ligation products== | ||
* Everything grew! Better efficiency with | * Everything grew! Better efficiency with electrop. than with chemical protocol | ||
==Single colony PCR to check our transformation== | ==Single colony PCR to check our transformation== | ||
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{|class="wikitable" style="text-align:center" border="1" | {|class="wikitable" style="text-align:center" border="1" | ||
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! Transformed products !! size of the product !! expected size after PCR | ! Transformed products !! size of the product (with cutting sites) !! expected size after PCR (about) | ||
|- | |- | ||
| Pupp || 255 || | | Pupp || 255 || 480 | ||
|- | |- | ||
| Pspac || 125 || | | Pspac || 125 || 350 | ||
|- | |- | ||
| RBS S || 56 || | | RBS S || 56 || 280 | ||
|- | |- | ||
| RBS W || 56 || | | RBS W || 56 || 280 | ||
|- | |- | ||
| agrD || 200 || | | agrD || 200 || 430 | ||
|} | |} | ||
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- run again on a e-gel | - run again on a e-gel | ||
* Lane1 :ladder 100bp | |||
* Lane2 : Pupp colony 1 | |||
* Lane3 : Pupp colony 3 | |||
* Lane4 : Pspac colony 1 | |||
* Lane5 : Pspac colony 3 | |||
* Lane6 : RBS S colony 1 | |||
* Lane7 : RBS S colony 4 | |||
* Lane8 : RBS W colony 1 | |||
* Lane9 : RBS W colony 4 | |||
* Lane10 : agrD colony 1 | |||
* Lane11 : agrD colony 4 | |||
* Lane12 : HyperladderI | |||
[[Image:photor.gif|300px|center]] | |||
*Result : nothing, just the primers! Problem with our PCR | *Result : nothing, just the primers! Problem with our PCR | ||
==Plate biobricks from MIT== | |||
*E0840 | |||
*B0014 | |||
*I712007 | |||
*C0012 | |||
*B0015 | |||
*C0061 | |||
*R0063 | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
Revision as of 09:09, 5 September 2008
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||
Results of transformation with our ligation products
Single colony PCR to check our transformation
- Single colony PCR : 13μL of SDW+cells, 5μL of Master Mix, 1μL of VF and 1μL of VR (and plate each single colony) - Load a gel (1.3% agarose) : 5μL of PCR products + 1μL of dye (only 1μL of 100b ladder) In the death plasmid, the VF-VR size is about 280b.
- run again on a e-gel
Plate biobricks from MIT
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