IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/02: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Line 66: | Line 66: | ||
- Gel (3% of agarose) | - Gel (3% of agarose) | ||
* Result | |||
{|class="wikitable" style="text-align:center" border="1" | |||
|- | |||
! gel !! lane !! inserted product (name of the colony) !! observation !! conclusion | |||
|- | |||
| top || 3 || RBS S (1) || one band (260bp) || size of the insert, RBS too small to see on a gel | |||
|- | |||
| top || 4 || RBS S (2) || one band (260bp) || size of the insert, RBS too small to see on a gel | |||
|- | |||
| top || 5 || RBS S (4) || nothing || ? | |||
|- | |||
| top || 6 || RBS S (5) || nothing || ? | |||
|- | |||
| top || 7 || RBS W (1) || 2 band (260bp + 350bp) || size of the insert, RBS too small to see on a gel + something else? | |||
|- | |||
| top || 8 || RBS W (2) || one band (350bp) || too big | |||
|- | |||
| top || 9 || RBS W (4) || one band (260bp) || size of the insert, RBS too small to see on a gel | |||
|- | |||
| top || 10 || RBS W (5) || one band (260bp) || size of the insert, RBS too small to see on a gel | |||
|- | |||
| top || 11 || Pspac (1) || 2 band (260bp + 350bp) || maybe problem with products loaded on gel (exactly the same bands than for RBS W) | |||
|- | |||
| top || 12 || Pspac (2) || one band (450bp) || size of Pupp? | |||
|- | |||
| top || 13 || Pspac (3) || one band (450bp) || size of Pupp? | |||
|- | |||
| bottom || 3 || Pspac (4) || nothing || ? | |||
|- | |||
| bottom || 4 || Pupp (1) || one band (400bp) || size of Pspac? | |||
|- | |||
| bottom || 5 || Pupp (2) || one band (400bp) || size of Pspac? | |||
|- | |||
| bottom || 6 || Pupp (3) || one band (slightly lower than 400bp) || size of Pspac? | |||
|- | |||
| bottom || 7 || Pupp (4) || one band (400bp) || size of Pspac? | |||
|- | |||
| bottom || 8 || agrD (1) || 2 bands (380 and 450bp) || big band is ok | |||
|- | |||
| bottom || 9 || agrD (2) || 2 bands (380 and 450bp) || big band is ok | |||
|- | |||
| bottom || 10 || agrD (3) || no bands || ? | |||
|- | |||
| bottom || 11 ||agrD (4) || strong band (450bp) || ok | |||
|- | |||
| bottom || 12 || P2 || one band (350bp) || ok | |||
|- | |||
|} | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> |
Revision as of 02:33, 4 September 2008
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Transformation of Bacillus
- IA751 on Cm5 and Spc 50 (control DNA less plates) - IA751 + ECE112 (1μg of DNA) on Cm5 - IA751 + ECE153 (0.6μg of DNA) on Scp50 - IA771 on Cm5 (control) - IA771 + ECE166 on Cm5
Check transformation in E.coli of our ligated products- Single colony PCR with VF and VR : add 10μL of SDW, 5μL of MM, 1μL of VF, 1μL of VR and 3μL of cells
- Gel (3% of agarose)
|