IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/02: Difference between revisions
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==Check ECE112 stock== | |||
- Double digest with XbaI and EcoRI | |||
- Gel : problem only one band (single digest???) | |||
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Revision as of 01:23, 5 September 2008
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Transformation of Bacillus
- IA751 on Cm5 and Spc 50 (control DNA less plates) - IA751 + ECE112 (1μg of DNA) on Cm5 - IA751 + ECE153 (0.6μg of DNA) on Scp50 - IA771 on Cm5 (control) - IA771 + ECE166 on Cm5
Check transformation in E.coli of our ligated products- Single colony PCR with VF and VR : add 10μL of SDW, 5μL of MM, 1μL of VF, 1μL of VR and 3μL of cells
- Gel (3% of agarose)
Check ECE112 stock- Double digest with XbaI and EcoRI - Gel : problem only one band (single digest???) |