IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/04: Difference between revisions
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==Result of Bacillus transformation (glycerol stock of competent cells)== | ==Result of Bacillus transformation (glycerol stock of competent cells)== | ||
{|class="wikitable" style="text-align:center" border="1" | |||
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! strain !! antibiotic !! added vector !! observation !! conclusion | |||
|- | |||
| IA751 || none || none || confluent colonies || cells alive | |||
|- | |||
| IA751 || Spc50 || none || nothing || no contamination | |||
|- | |||
| IA751 || Spc50 || ECE153 || 15 || ok, efficiency 25 colonies/μg of DNA | |||
|- | |||
| IA771 || none || none || confluent colonies || cells alive | |||
|- | |||
| IA771 || Cm5 || none || nothing || no contamination | |||
|- | |||
| IA771 || Cm5 || ECE166 || 15 || ok, efficiency 5 colonies/μg of DNA | |||
|} | |||
We have no contamination in our glycerol stocks. However, the efficiency of glycerol stocks seems to be lower than these of fresh competent cells. | |||
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Revision as of 02:18, 5 September 2008
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Result of Bacillus transformation (glycerol stock of competent cells)
We have no contamination in our glycerol stocks. However, the efficiency of glycerol stocks seems to be lower than these of fresh competent cells. | ||||||||||||||||||||||||||||||||||||
