IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/04: Difference between revisions

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We have no contamination in our glycerol stocks. However, the efficiency of glycerol stocks seems to be lower than these of fresh competent cells.
We have no contamination in our glycerol stocks. However, the efficiency of glycerol stocks seems to be lower than these of fresh competent cells.
==Results of new "biobricks"transformation==
- Nothing on our plate, except for the control plate.
==New attempt of new "biobricks" transformation==


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Revision as of 02:19, 5 September 2008



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Result of Bacillus transformation (glycerol stock of competent cells)

strain antibiotic added vector observation conclusion
IA751 none none confluent colonies cells alive
IA751 Spc50 none nothing no contamination
IA751 Spc50 ECE153 15 ok, efficiency 25 colonies/μg of DNA
IA771 none none confluent colonies cells alive
IA771 Cm5 none nothing no contamination
IA771 Cm5 ECE166 15 ok, efficiency 5 colonies/μg of DNA

We have no contamination in our glycerol stocks. However, the efficiency of glycerol stocks seems to be lower than these of fresh competent cells.

Results of new "biobricks"transformation

- Nothing on our plate, except for the control plate.

New attempt of new "biobricks" transformation