IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/11: Difference between revisions

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*Result
*Result


[[Image:gel11092pcr.gif|200px|center]]
[[Image:gel11092pcr.gif|300px|center]]


- Nothing, except for the PCR with VF and VR primers, either our primers are not right, either we have no insert.
- Nothing, except for the PCR with VF and VR primers, either our primers are not right, either we have no insert.

Revision as of 01:49, 16 September 2008

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RBS screening

- We run our PCR products from yesterday on a new 1.8% agarose gel

- Gel1

  • Lane2 : RBSS1
  • Lane3 : RBS S2
  • Lane4 : RBS S3
  • Lane5 : RBS S4
  • Lane6 : RBS S5
  • Lane7 : RBS S6
  • Lane8 : HyperladderIV
  • Lane9 : RBS S7
  • Lane10 : RBS S8
  • Lane11 : RBS S9
  • Lane12 : RBS S10
  • Lane13 : RBS S6 with VF and VR primers
  • Lane14 : RBS S11

- Gel2

  • Lane2 : RBSW1
  • Lane3 : RBS W2
  • Lane4 : RBS W3
  • Lane5 : RBS W4
  • Lane6 : RBS W5
  • Lane7 : RBS W6
  • Lane8 : HyperladderIV
  • Lane9 : RBS W7
  • Lane10 : RBS W8
  • Lane11 : RBS W9
  • Lane12 : RBS W10
  • Lane13 : RBS W6 with VF and VR primers
  • Lane14 : RBS W11
  • Result

- Nothing, except for the PCR with VF and VR primers, either our primers are not right, either we have no insert.



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