IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/11
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< IGEM:Cambridge | 2008 | Notebook | Turing Pattern Formation | 2008 | 09(Difference between revisions)
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Current revision (05:06, 16 September 2008) (view source) (→RBS screening) |
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- Nothing, except for the PCR with VF and VR primers, either our primers are not right, either we have no insert. | - Nothing, except for the PCR with VF and VR primers, either our primers are not right, either we have no insert. | ||
| + | ==RBS screning but single digest== | ||
| + | |||
| + | - Grow some colonies of Top10 transformed with RBS into 10mL of LB with antibiotic | ||
| + | |||
| + | We want to check our transformation with single digest. If we have self ligation in our transformation, we will loose the XbaI cutting site. | ||
| + | |||
| + | ==Backbone for ligation== | ||
| + | |||
| + | - New pellets in 600μL of SDW | ||
| + | |||
| + | - Plasmid miniprep | ||
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RBS screening- We run our PCR products from yesterday on a new 1.8% agarose gel - Gel1
- Gel2
 - Nothing, except for the PCR with VF and VR primers, either our primers are not right, either we have no insert. RBS screning but single digest- Grow some colonies of Top10 transformed with RBS into 10mL of LB with antibiotic We want to check our transformation with single digest. If we have self ligation in our transformation, we will loose the XbaI cutting site. Backbone for ligation- New pellets in 600μL of SDW - Plasmid miniprep | |
