IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13: Difference between revisions
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- Single digest with EcoRI and XbaI | - Single digest with EcoRI and XbaI | ||
- Gel1 | |||
*Lane2 : HyperladderI | |||
*Lane3 : RBS W2 cut with EcoRI | |||
*Lane4 : RBS W2 cut with XbaI | |||
*Lane5 : RBS W4 cut with EcoRI | |||
*Lane6 : RBS W4 cut with XbaI | |||
*Lane7 : RBS W5 cut with EcoRI | |||
*Lane8 : RBS W5 cut with XbaI | |||
*Lane9 : RBS W6 cut with EcoRI | |||
*Lane10 : RBS W6 cut with XbaI | |||
*Lane11 : RBS W8 uncut | |||
*Lane12 : supercoiled ladder | |||
- Gel2 | |||
*Lane2 : HyperladderI | |||
*Lane3 : RBS W8 cut with EcoRI | |||
*Lane4 : RBS W8 cut with XbaI | |||
*Lane5 : RBS W10 cut with EcoRI | |||
*Lane6 : RBS W10 cut with XbaI | |||
*Lane7 : RBS W11 cut with EcoRI | |||
*Lane8 : RBS W11 cut with XbaI | |||
*Lane9 : RBS W8 uncut | |||
*Lane10 : supercoiled ladder | |||
*Result | |||
[[Image:gel31091pcr.gif|250px|center]] | |||
- W2 with EcoRI : cut | |||
- W2 with XbaI : uncut, self ligation | |||
- W4 with EcoRI : cut | |||
- W4 with XbaI : uncut, self ligation | |||
- W5 with EcoRI : cut | |||
- W5 with XbaI : uncut, self ligation | |||
- W6 with EcoRI : cut | |||
- W6 with XbaI : cut (2 bands), transformation ok for RBS W6 | |||
- W8 with EcoRI : impossible to see | |||
- W8 with XbaI : impossible to see | |||
- W10 with EcoRI : cut | |||
- W10 with XbaI : maybe cut, it seems to be 2 bands, but quite difficult to see | |||
- W11 with EcoRI : cut | |||
- W11 with XbaI : uncut, self ligation | |||
==Double check of RBS W6 and stock== | |||
- Grow RBS W6 in 10mL of LB with antibiotic | |||
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Revision as of 03:06, 16 September 2008
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Grow RBS S6- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic - Incubate at 37°C with shaking RBS W screening- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer) - Nanodrop
- Single digest with EcoRI and XbaI - Gel1
- W2 with EcoRI : cut - W2 with XbaI : uncut, self ligation - W4 with EcoRI : cut - W4 with XbaI : uncut, self ligation - W5 with EcoRI : cut - W5 with XbaI : uncut, self ligation - W6 with EcoRI : cut - W6 with XbaI : cut (2 bands), transformation ok for RBS W6 - W8 with EcoRI : impossible to see - W8 with XbaI : impossible to see - W10 with EcoRI : cut - W10 with XbaI : maybe cut, it seems to be 2 bands, but quite difficult to see - W11 with EcoRI : cut - W11 with XbaI : uncut, self ligation Double check of RBS W6 and stock- Grow RBS W6 in 10mL of LB with antibiotic |