IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Entry title)
(RBS W screening)
Line 22: Line 22:
- Nanodrop
- Nanodrop
 +
 +
{|class="wikitable" style="text-align:center" border="1"
 +
|-
 +
! product !! concentration (ng/μL) !! 260/280 !! quantity of DNA to add to have about 300ng (μL) !! added SDW for single digest (μL)
 +
|-
 +
| RBS W2 || 42.4 || 1.62 || 8 || 9
 +
|-
 +
| RBS W4 || 35.1 || 1.61 || 10 || 7
 +
|-
 +
| RBS W6 || 41.0 || 1.67 || 8 || 9
 +
|-
 +
| RBS W8 || 72.8 || 1.61 || 5 || 12
 +
|-
 +
| RBS W10 || 51.0 || 1.60 || 6 || 11
 +
|}
 +

Revision as of 04:51, 16 September 2008



Main project page
Previous entry      Next entry

Grow RBS S6

- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic

- Incubate at 37°C with shaking

RBS W screening

- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer)

- Nanodrop

product concentration (ng/μL) 260/280 quantity of DNA to add to have about 300ng (μL) added SDW for single digest (μL)
RBS W2 42.4 1.62 8 9
RBS W4 35.1 1.61 10 7
RBS W6 41.0 1.67 8 9
RBS W8 72.8 1.61 5 12
RBS W10 51.0 1.60 6 11




Personal tools