IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13
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| + | ! product !! concentration (ng/μL) !! 260/280 !! quantity of DNA to add to have about 300ng (μL) !! added SDW for single digest (μL) | ||
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| + | | RBS W2 || 42.4 || 1.62 || 8 || 9 | ||
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| + | | RBS W4 || 35.1 || 1.61 || 10 || 7 | ||
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| + | | RBS W6 || 41.0 || 1.67 || 8 || 9 | ||
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| + | | RBS W8 || 72.8 || 1.61 || 5 || 12 | ||
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| + | | RBS W10 || 51.0 || 1.60 || 6 || 11 | ||
| + | |} | ||
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Revision as of 05:51, 16 September 2008
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Grow RBS S6- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic - Incubate at 37°C with shaking RBS W screening- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer) - Nanodrop
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