IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13

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- Single digest with EcoRI and XbaI

Revision as of 05:52, 16 September 2008



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Grow RBS S6

- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic

- Incubate at 37°C with shaking

RBS W screening

- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer)

- Nanodrop

product concentration (ng/μL) 260/280 quantity of DNA to add to have about 300ng (μL) added SDW for single digest (μL)
RBS W2 42.4 1.62 8 9
RBS W4 35.1 1.61 10 7
RBS W6 41.0 1.67 8 9
RBS W8 72.8 1.61 5 12
RBS W10 51.0 1.60 6 11

- Single digest with EcoRI and XbaI



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