IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/13: Difference between revisions
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- Single digest with EcoRI and XbaI | - Single digest with EcoRI and XbaI | ||
- Gel1 | |||
*Lane2 : HyperladderI | |||
*Lane3 : RBS W2 cut with EcoRI | |||
*Lane4 : RBS W2 cut with XbaI | |||
*Lane5 : RBS W4 cut with EcoRI | |||
*Lane6 : RBS W4 cut with XbaI | |||
*Lane7 : RBS W5 cut with EcoRI | |||
*Lane8 : RBS W5 cut with XbaI | |||
*Lane9 : RBS W6 cut with EcoRI | |||
*Lane10 : RBS W6 cut with XbaI | |||
*Lane11 : RBS W8 uncut | |||
*Lane12 : supercoiled ladder | |||
- Gel2 | |||
*Lane2 : HyperladderI | |||
*Lane3 : RBS W8 cut with EcoRI | |||
*Lane4 : RBS W8 cut with XbaI | |||
*Lane5 : RBS W10 cut with EcoRI | |||
*Lane6 : RBS W10 cut with XbaI | |||
*Lane7 : RBS W11 cut with EcoRI | |||
*Lane8 : RBS W11 cut with XbaI | |||
*Lane9 : RBS W8 uncut | |||
*Lane10 : supercoiled ladder | |||
*Result | |||
[[Image:gel31091pcr.gif|250px|center]] | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> |
Revision as of 02:54, 16 September 2008
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Grow RBS S6- Put the 10mL of LB with RBS S6 from yesterday into 200mL of LB with antibiotic - Incubate at 37°C with shaking RBS W screening- Plasmid miniprep of RBS W2, 4, 6, 8, 10 9witouh endo wash buffer) - Nanodrop
- Single digest with EcoRI and XbaI - Gel1
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