IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/15
From OpenWetWare
(Difference between revisions)
(→Entry title) |
(→Check big stock of RBS S and W) |
||
| Line 17: | Line 17: | ||
- Nanodrop | - Nanodrop | ||
| + | |||
| + | {|class="wikitable" style="text-align:center" border="1" | ||
| + | |- | ||
| + | ! product !! concentration (ng/μL) !! 260/280 | ||
| + | |- | ||
| + | | RBS W2 || 90.3 || 1.70 | ||
| + | |- | ||
| + | | RBS W4 || 124.0 || 1.80 | ||
| + | |} | ||
| + | |||
| + | - Single digest with EcoRI, XbaI, SpeI and PstI | ||
| + | |||
| + | - Gel | ||
| + | |||
| + | *Lane1 : HyperladderI | ||
| + | *Lane2 : RBS S6 cut with EcoRI | ||
| + | *Lane3 : RBS S6 cut with SpeI | ||
| + | *Lane4 : RBS S6 cut with PstI | ||
| + | *Lane5 : RBS S6 cut with XbaI | ||
| + | *Lane6 : RBS S6 uncut | ||
| + | *Lane7 : RBS W6 cut with EcoRI | ||
| + | *Lane8 : RBS W6 cut with SpeI | ||
| + | *Lane9 : RBS W6 cut with PstI | ||
| + | *Lane10 : RBS W6 cut with XbaI | ||
| + | *Lane11 : RBS W6 uncut | ||
| + | *Lane12 : supercoiled ladder | ||
| + | |||
| + | *Result | ||
| + | |||
| + | |||
Revision as of 06:20, 16 September 2008
 |  Main project page Previous entry
| |||||||||
Check big stock of RBS S and W
- Plasmid miniprep RBS S6 and RBS W6 (from 200mL flask) - Nanodrop
- Single digest with EcoRI, XbaI, SpeI and PstI - Gel
| ||||||||||
