IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/09/15: Difference between revisions
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- Even no band for the uncut plasmid. There is a problem with plasmid miniprep, we will try again with less (more diluted) cells for plasmid miniprep. | |||
- New plasmid miniprep (the pellets from 20mL of LB is diluted into 1.2ml instead of 0.6) | |||
- New single digest | |||
- New gel (same lanes than before) | |||
*Result | |||
[[Image:gel51092pcr.gif|250px|center]] | |||
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Revision as of 03:24, 16 September 2008
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html> | |||||||||
Check big stock of RBS S and W
- Plasmid miniprep RBS S6 and RBS W6 (from 200mL flask) - Nanodrop
- Single digest with EcoRI, XbaI, SpeI and PstI - Gel
 - Even no band for the uncut plasmid. There is a problem with plasmid miniprep, we will try again with less (more diluted) cells for plasmid miniprep. - New plasmid miniprep (the pellets from 20mL of LB is diluted into 1.2ml instead of 0.6) - New single digest - New gel (same lanes than before)
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