IGEM:Cambridge/2008/Notebook/Voltage/2008/07/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
(Autocreate 2008/07/22 Entry for IGEM:Cambridge/2008/Notebook/Voltage) |
|||
| Line 5: | Line 5: | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
== | ==Wet Work== | ||
* | * Inoculated 10mL LB with E.coli strain MG1655 | ||
* Inoculated 10mL LB with E.coli strain TOP10 transformed with BioBrick J5311 | |||
16 LA plates were poured with 8 having 25μg/mL Kanamycin, ready for mutant strains to be grown on. | |||
The gel extracts from the ligation of primer and rbs were extracted using Zymoclean gel extraction kit. Hopefully these now contain linear DNA of BBa_J23113 and BBa_B0030, and BBa_J23114 and BBa_B0030. | |||
BioBricks J45996, J45995 and J45250 were also extracted from the registry, using the "bigger, better, faster, stronger, method", in order to provide template to extracted the OsmY promoter. | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> | ||
__NOTOC__ | __NOTOC__ | ||
Revision as of 08:38, 23 July 2008
 ........... Electric Output
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Wet Work
16 LA plates were poured with 8 having 25μg/mL Kanamycin, ready for mutant strains to be grown on. The gel extracts from the ligation of primer and rbs were extracted using Zymoclean gel extraction kit. Hopefully these now contain linear DNA of BBa_J23113 and BBa_B0030, and BBa_J23114 and BBa_B0030. BioBricks J45996, J45995 and J45250 were also extracted from the registry, using the "bigger, better, faster, stronger, method", in order to provide template to extracted the OsmY promoter.
|
|
