IGEM:Cambridge/2008/Notebook/Voltage/2008/07/23: Difference between revisions

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(Autocreate 2008/07/23 Entry for IGEM:Cambridge/2008/Notebook/Voltage)
 
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==Entry title==
==Wet Work==
* Insert your content here.
* Recovered Biobrick plasmid YFP from previous culture.
**1.6 ml of cells according to Zymogen Plasmid Prep Kit protocol (60μL Elution Buffer used)
 
*Added 20ml LB to 10ml innoculation of E Coli MG1655 (see 22/07/08) to increase volume in preparation for experiments tomorrow on K+ tolerance in media.






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Revision as of 08:26, 23 July 2008

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Wet Work

  • Recovered Biobrick plasmid YFP from previous culture.
    • 1.6 ml of cells according to Zymogen Plasmid Prep Kit protocol (60μL Elution Buffer used)
  • Added 20ml LB to 10ml innoculation of E Coli MG1655 (see 22/07/08) to increase volume in preparation for experiments tomorrow on K+ tolerance in media.