IGEM:Cambridge/2008/Notebook/Voltage/2008/07/29: Difference between revisions

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* E-Gel (SYBR) to confirm OsmY
* E-Gel (SYBR) to confirm OsmY
* E-Gel (EtBr) to confirm Kdp and OsmY
* E-Gel (EtBr) to confirm Kdp and OsmY
[[Image:1st Gel OsmY KDP PCR.jpg|frame|left|Gel showing KDP (2 thick bands on the left and OsmY (dimmer bands to the right)]]




Revision as of 15:54, 31 July 2008

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  • Mutant E.coli strains had grown on plates.
  • Transferred mutants to liquid culture.
  • Primers arrived! Prepped as described in Protocols page
  • PCR started with OsmY (from Biobrick plasmids) and KDP (from E. Coli MG1655 Cell Culture)
  • E-Gel (SYBR) to confirm OsmY
  • E-Gel (EtBr) to confirm Kdp and OsmY
Gel showing KDP (2 thick bands on the left and OsmY (dimmer bands to the right)



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