IGEM:Cambridge/2008/Notebook/Voltage/2008/07/31: Difference between revisions

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(Autocreate 2008/07/31 Entry for IGEM:Cambridge/2008/Notebook/Voltage)
 
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==Entry title==
==Mutants==
* Insert your content here.
* Mutant strains were grown in different potassium levels: 1mM, 50mM and 200mM.
* Growth curves were plotted, using OD600 readings every 30 minutes for 3 hours.


==Kdp==
* Ligated Kdp into death vector PSB4C5 again.
==OsmY==
* TOP10 containing osmY-containing plasmid were grown in LB.




Revision as of 08:36, 1 August 2008

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Mutants

  • Mutant strains were grown in different potassium levels: 1mM, 50mM and 200mM.
  • Growth curves were plotted, using OD600 readings every 30 minutes for 3 hours.

Kdp

  • Ligated Kdp into death vector PSB4C5 again.

OsmY

  • TOP10 containing osmY-containing plasmid were grown in LB.



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