IGEM:Cambridge/2008/Notebook/Voltage/2008/08/04: Difference between revisions
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==Biobricks== | μL==Biobricks== | ||
* The liagtion reaction from last week was drop dialised and then 5μL used to transform highly competent TOP10, before plating on chloramphenicol plates. | * The liagtion reaction from last week was drop dialised and then 5μL used to transform highly competent TOP10, before plating on chloramphenicol plates. | ||
* The Biobrick combination of promoter-RBS were then cut with Pst1 and EcoR1 and ligated into pSB4C5 and then transformed into TOP10 and | * The Biobrick combination of promoter-RBS were then cut with Pst1 and EcoR1 and ligated into pSB4C5 and then transformed into TOP10 and incubated overnight at room temperature in 600μL SOC. | ||
Revision as of 11:56, 4 August 2008
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μL==Biobricks==
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