IGEM:Cambridge/2008/Notebook/Voltage/2008/08/11: Difference between revisions

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(Autocreate 2008/08/11 Entry for IGEM:Cambridge/2008/Notebook/Voltage)
 
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==Entry title==
==Kdp?==
* Insert your content here.
*PCR colonies on plates - no insert seen.
* Transform overweekend ligation.
 
==OsmY and RBS==
 
* PCR B0030 and restrict 10μL with Xba.
* Plasmid extract OsmY and restriction digest with Xba.
* Ligate and transform cells with 5μL




Revision as of 06:08, 14 August 2008

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Kdp?

  • PCR colonies on plates - no insert seen.
  • Transform overweekend ligation.

OsmY and RBS

  • PCR B0030 and restrict 10μL with Xba.
  • Plasmid extract OsmY and restriction digest with Xba.
  • Ligate and transform cells with 5μL



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