IGEM:Cambridge/2008/Notebook/Voltage/2008/08/12: Difference between revisions
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(Autocreate 2008/08/12 Entry for IGEM:Cambridge/2008/Notebook/Voltage) |
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== | ==KDP== | ||
* | * Again try ligation of Kdp and Vector backbone | ||
==OsmY== | |||
* Again try ligation of OsmY and B0030 backbone | |||
* This time try using Xba for B0030 and ligate. Gel again and cut out linear fragment. Cut with Spe and gel - cut out circular fragment. | |||
==Make competent stocks== | |||
Chemically competent TOP10 cells were made by growing overnight in 200ml LB in a shaking incubater at 37°C. | |||
200ml were spun down at 3800rpm for 8 mins, resuspended in 20ml CaCl<sup>2</sup>, spun down again and resuspended in 4ml CaCl<sup>2</sup>. | |||
Revision as of 06:15, 14 August 2008
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KDP
OsmY
Make competent stocksChemically competent TOP10 cells were made by growing overnight in 200ml LB in a shaking incubater at 37°C. 200ml were spun down at 3800rpm for 8 mins, resuspended in 20ml CaCl2, spun down again and resuspended in 4ml CaCl2.
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