IGEM:Cambridge/2008/Notebook/Voltage/2008/08/13: Difference between revisions
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(Autocreate 2008/08/13 Entry for IGEM:Cambridge/2008/Notebook/Voltage) |
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* | * Same ole, same ole. | ||
==Recovering frozen cells== | |||
To recover frozen cells they were thawed on ice and spun down at6500rpm for 5mins. They were then resuspended in 100μL CaCl<sub>2</sub>. | |||
They were then transformed with 2μL pUC9 and grown on 100μg/mL Amp plates. | |||
Revision as of 06:24, 14 August 2008
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Kdp
Recovering frozen cellsTo recover frozen cells they were thawed on ice and spun down at6500rpm for 5mins. They were then resuspended in 100μL CaCl2. They were then transformed with 2μL pUC9 and grown on 100μg/mL Amp plates.
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