IGEM:Cambridge/2008/Notebook/Voltage/2008/08/26
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==Kdp screening== | ==Kdp screening== | ||
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* Single colony PCR was perfomed over a selection of 44 colonies using forward and reverse biobrick primers in order to gauge the size of the vector insert by gel visualisation. | * Single colony PCR was perfomed over a selection of 44 colonies using forward and reverse biobrick primers in order to gauge the size of the vector insert by gel visualisation. | ||
*All screened colonies were negative for Kdp - concluded most likely to be blunt ended self ligation of pSB4C5 vector (without death gene). | *All screened colonies were negative for Kdp - concluded most likely to be blunt ended self ligation of pSB4C5 vector (without death gene). | ||
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