IGEM:Cambridge/2008/Notebook/Voltage/K+ Growth
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Method
- For each of the 9 mutants, 3 [K+] of growth medium were examined: 1mM, 50mM & 200mM
- Cuvette composition * 100ul cells
* 100ul KCl (10 times more concentrated stock to make up to correct conc.) * 800ul LB
- The spectrophotometer was zeroed using LB.
- OD readings were taken every 30 minutes for 3 hours.
- In between readings, cultures were stored at room temperature & covered with parafilm.