IGEM:Cambridge/2008/Notebook/Voltage/OD600 Calibration: Difference between revisions
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=Method= | =Method= | ||
* | *An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.) | ||
* | *Dilutions were plated out at 10<sup>-5</sup> and 10<sup>-6</sup> fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions. | ||
=Results= | =Results= | ||
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{| border="1" cellpadding="2" | {| border="1" cellpadding="2" | ||
|-valign="top" | |-valign="top" | ||
|width="20%"|'''Relative Cell Density''' |width="20%"|'''OD600 Reading''' | |width="20%"|'''Relative Cell Density''' | ||
|width="20%"|'''OD600 Reading''' | |||
|width="20%"|'''Colonies on x100,000 dilution''' | |||
|-valign="top" | |-valign="top" | ||
|width="20%"|1 | |width="20%"|1 | ||
|width="20%"|1.6483 | |width="20%"|1.6483 | ||
|width="20%"|745 | |||
|-valign="top" | |-valign="top" | ||
|width="20%"|0.8 | |width="20%"|0.8 | ||
| Line 19: | Line 22: | ||
|-valign="top" | |-valign="top" | ||
|width="20%"|0.4 | |width="20%"|0.4 | ||
|width="20%"|1.0167 | |width="20%"|1.0167 | ||
|width="20%"|308 | |||
|-valign="top" | |-valign="top" | ||
|width="20%"|0.2 | |width="20%"|0.2 | ||
Latest revision as of 07:02, 3 September 2008
Method
- An LB culture of TOP10 cells was serially diluted and the OD600 reading was taken for each. (UV/Vis Spectrophotometer zeroed using LB.)
- Dilutions were plated out at 10-5 and 10-6 fold dilutions, and colonies were counted using a colony counter to determine the total number of cells in the original dilutions.
Results
Relative Cell Density OD600 Reading Colonies on x100,000 dilution 1 1.6483 745 0.8 1.4913 0.6 1.1058 0.4 1.0167 308 0.2 0.5885 0.1 0.2982 0.08 0.2567 0.06 0.1910 0.04 0.1208 0.02 0.070 0.01 0.0346
