IGEM:Cambridge/2008/Notebook/Voltage/Output

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< IGEM:Cambridge | 2008 | Notebook | Voltage(Difference between revisions)
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Current revision (07:26, 28 October 2008) (view source)
 
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=Setup=
=Setup=
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Cells containing biobrick K090002 are grown overnight in [[IGEM:Cambridge/2008/Protocols#NOK_2.0_.28No_potassium_nutrient_broth.29| NOK]]
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Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.
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*Cells containing biobrick K090002 are grown overnight in [[IGEM:Cambridge/2008/Protocols#NOK_2.0_.28No_potassium_nutrient_broth.29| NOK]] with 5%(by vol.) 2M KCl added.
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*Samples are then spun down in individual 1.5ml eppendorfs.
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=Experiment=
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*Resuspend cells in 200μL clean NOK and place in sample wells
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*Lower probe electrode into cell solution and wait for stable reading - 1min.
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*Add sample solution

Current revision

Setup

Apparatus is setup as shown. An earth is needed next to the probe electrode, but not touching. They will be needed within the same solution to reduce the noise.

  • Cells containing biobrick K090002 are grown overnight in NOK with 5%(by vol.) 2M KCl added.
  • Samples are then spun down in individual 1.5ml eppendorfs.


Experiment

  • Resuspend cells in 200μL clean NOK and place in sample wells
  • Lower probe electrode into cell solution and wait for stable reading - 1min.
  • Add sample solution
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