IGEM:Cambridge/2008/PresentationOutline: Difference between revisions
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==Voltage== | ==Introduction (Linda)== | ||
==Voltage (Ellis)== | |||
*KDP | *KDP | ||
**K+ ATPase, import pump | **K+ ATPase, import pump | ||
| Line 16: | Line 17: | ||
**Pictures | **Pictures | ||
==Peptide Signaling== | ==Peptide Signaling (Daniel)== | ||
*AGR System | *AGR System | ||
**Sender & Reciever | **Sender & Reciever | ||
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**Requires expression of membrane channels | **Requires expression of membrane channels | ||
==Bacillus== | ==Bacillus(Daniel)== | ||
*About Bacillus | *About Bacillus | ||
**Pretty Pictures | **Pretty Pictures | ||
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*New GFP | *New GFP | ||
**[http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP ''Stefan's page''] | **[http://openwetware.org/wiki/IGEM:Cambridge/2008/Improved_GFP ''Stefan's page''] | ||
==Modeling== | ==Modeling (Xiao-hu)== | ||
*The agr-peptide signalling system. | *The agr-peptide signalling system. | ||
Latest revision as of 10:15, 26 October 2008
Introduction (Linda)
Voltage (Ellis)
- KDP
- K+ ATPase, import pump
- PCRed from genome
- OsmY
- Flame Photometry
- Data and graphs
- GluR0
- Glutamate gated K+ channel
- From Synechosystis
- Constitutive Promoter
- Voltage Output
- Pictures
Peptide Signaling (Daniel)
- AGR System
- Sender & Reciever
- Compared to Lux
- More robust, specific
- Controlled secretion, not membrane diffusion
- Multichannel signaling (no crosstalk)
- Requires expression of membrane channels
Bacillus(Daniel)
- About Bacillus
- Pretty Pictures
- Best-characterized Gram Positive
- Very safe
- Genome Sequenced
- Protein Secretion
- Not compatible with Gram-neg promoters/RBS
Techniques/Tools:
- Chromosomal Integration
- Single Copy
- Cool diagram: single vs. double x-over
- Detection: amylase assay & PCR
- InFusion
- New Vectors
- Bacillus RBS & Promoters
- Two Constituitive, Two Inducible
- Culled from existing vectors
- Picture of Bacillus expressing GFP with pUpp
- New GFP
Modeling (Xiao-hu)
- The agr-peptide signalling system.
- Assumptions.
- Results: Step-like behaviour, system highly sensitive to basal rates of expression.
- Pattern formation.
- Some simple models.
- Patterns with agr-quorum sensing systems: competing populations.
- Further questions: Concise models, spatial pattern generation with a single signalling molecule.
