IGEM:Cambridge/2008/PresentationOutline

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==Voltage==
==Voltage==
 +
*KDP
 +
**K+ ATPase, import pump
 +
**PCRed from genome
 +
**OsmY
 +
 +
*Flame Photometry
 +
**Data and graphs
 +
 +
*GluR0
 +
**Glutamate gated K+ channel
 +
**From Synechosystis
 +
**Constitutive Promoter
 +
 +
*Voltage Output
 +
**Pictures
 +
==Peptide Signaling==
==Peptide Signaling==
*AGR System
*AGR System

Revision as of 09:42, 26 October 2008

Contents

Voltage

  • KDP
    • K+ ATPase, import pump
    • PCRed from genome
    • OsmY
  • Flame Photometry
    • Data and graphs
  • GluR0
    • Glutamate gated K+ channel
    • From Synechosystis
    • Constitutive Promoter
  • Voltage Output
    • Pictures

Peptide Signaling

  • AGR System
    • Sender & Reciever
  • Compared to Lux
    • More robust, specific
    • Controlled secretion, not membrane diffusion
    • Multichannel signaling (no crosstalk)
    • Requires expression of membrane channels

Bacillus

  • About Bacillus
    • Pretty Pictures
    • Best-characterized Gram Positive
    • Very safe
    • Genome Sequenced
    • Protein Secretion
    • Not compatible with Gram-neg promoters/RBS

Techniques/Tools:

  • Chromosomal Integration
    • Single Copy
    • Cool diagram: single vs. double x-over
    • Detection: amylase assay & PCR
  • InFusion
  • New Vectors
  • Bacillus RBS & Promoters
    • Two Constituitive, Two Inducible
    • Culled from existing vectors
    • Picture of Bacillus expressing GFP with pUpp
  • New GFP

Modeling

Personal tools