IGEM:Cambridge/2008/PresentationOutline: Difference between revisions
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==Voltage== | ==Voltage== | ||
*KDP | |||
**K+ ATPase, import pump | |||
**PCRed from genome | |||
**OsmY | |||
*Flame Photometry | |||
**Data and graphs | |||
*GluR0 | |||
**Glutamate gated K+ channel | |||
**From Synechosystis | |||
**Constitutive Promoter | |||
*Voltage Output | |||
**Pictures | |||
==Peptide Signaling== | ==Peptide Signaling== | ||
*AGR System | *AGR System | ||
Revision as of 07:42, 26 October 2008
Voltage
- KDP
- K+ ATPase, import pump
- PCRed from genome
- OsmY
- Flame Photometry
- Data and graphs
- GluR0
- Glutamate gated K+ channel
- From Synechosystis
- Constitutive Promoter
- Voltage Output
- Pictures
Peptide Signaling
- AGR System
- Sender & Reciever
- Compared to Lux
- More robust, specific
- Controlled secretion, not membrane diffusion
- Multichannel signaling (no crosstalk)
- Requires expression of membrane channels
Bacillus
- About Bacillus
- Pretty Pictures
- Best-characterized Gram Positive
- Very safe
- Genome Sequenced
- Protein Secretion
- Not compatible with Gram-neg promoters/RBS
Techniques/Tools:
- Chromosomal Integration
- Single Copy
- Cool diagram: single vs. double x-over
- Detection: amylase assay & PCR
- InFusion
- New Vectors
- Bacillus RBS & Promoters
- Two Constituitive, Two Inducible
- Culled from existing vectors
- Picture of Bacillus expressing GFP with pUpp
- New GFP
