IGEM:Cambridge/2008/PresentationOutline

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< IGEM:Cambridge | 2008
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Contents

Introduction (Linda)

Voltage (Ellis)

  • KDP
    • K+ ATPase, import pump
    • PCRed from genome
    • OsmY
  • Flame Photometry
    • Data and graphs
  • GluR0
    • Glutamate gated K+ channel
    • From Synechosystis
    • Constitutive Promoter
  • Voltage Output
    • Pictures

Peptide Signaling (Daniel)

  • AGR System
    • Sender & Reciever
  • Compared to Lux
    • More robust, specific
    • Controlled secretion, not membrane diffusion
    • Multichannel signaling (no crosstalk)
    • Requires expression of membrane channels

Bacillus(Daniel)

  • About Bacillus
    • Pretty Pictures
    • Best-characterized Gram Positive
    • Very safe
    • Genome Sequenced
    • Protein Secretion
    • Not compatible with Gram-neg promoters/RBS

Techniques/Tools:

  • Chromosomal Integration
    • Single Copy
    • Cool diagram: single vs. double x-over
    • Detection: amylase assay & PCR
  • InFusion
  • New Vectors
  • Bacillus RBS & Promoters
    • Two Constituitive, Two Inducible
    • Culled from existing vectors
    • Picture of Bacillus expressing GFP with pUpp
  • New GFP

Modeling (Xiao-hu)

  • The agr-peptide signalling system.
    • Assumptions.
    • Results: Step-like behaviour, system highly sensitive to basal rates of expression.
  • Pattern formation.
    • Some simple models.
    • Patterns with agr-quorum sensing systems: competing populations.
  • Further questions: Concise models, spatial pattern generation with a single signalling molecule.
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