IGEM:Cambridge/2008/Turing Pattern Formation/Constructs: Difference between revisions
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Lowercase letters are biobrick ends, uppercase are the actual pieces. | Lowercase letters are biobrick ends, uppercase are the actual pieces. | ||
==AgrA== | |||
<pre>atgaattcgcggccgcttctagagATGGAAATCGCACTGGCTACGGACAATCCATATGAGGTTCTGGAACAGGCTAAAAACATGAACGACATCGGCTGCTATTTCCTGGATATTCAGCTGAGCACCGACATCAACGGTATCAAACTGGGCTCTGAAATCCGTAAACATGATCCGGTTGGTAACATTATTTTCGTGACTTCTCATTCTGAGCTGACCTATCTGACGTTCGTATACAAGGTTGCAGCCATGGATTTCATCTTTAAAGACGATCCGGCAGAGCTGCGTACGCGCATCATCGACTGTCTGGAAACTGCGCACACGCGCCTGCAACTGCTGTCCAAAGATAACTCCGTGGAAACCATTGAACTGAAACGTGGCAGCAACAGCGTTTACGTTCAGTACGATGATATCATGTTCTTCGAAAGCTCCACTAAGTCCCACCGTCTGATCGCGCATCTGGATAATCGTCAGATCGAGTTCTATGGCAACCTGAAAGAGCTGAGCCAGCTGGACGATCGCTTCTTCCGTTGTCATAACTCCTTCGTTGTTAACCGTCACAACATCGAATCCATCGACTCCAAAGAGCGTATTGTATACTTCAAGAACAAAGAACACTGTTATGCGTCTGTACGCAATGTCAAGAAAATCTAAtactagtagcggccgctgcaggcggc</pre> | |||
==AgrB== | |||
<pre>atgaattcgcggccgcttctagagATGAACTATTTTGACAACAAGATCGACCAATTTGCTACTTACCTGCAAAAACGCAACAACCTGGACCACATCCAGTTTCTGCAAGTTCGTCTGGGCATGCAGGTTCTGGCTAAAAACATCGGCAAACTGATCGTTATGTACACCATCGCCTATATTCTGAACATCTTCCTGTTTACCCTGATCACCAACCTGACCTTCTATCTGATTCGCCGTCATGCCCACGGCGCTCATGCACCTTCTTCCTTTTGGTGTTATGTAGAGAGCATTATTCTGTTTATCCTGCTGCCGCTGGTAATCGTTAACTTCCACATCAATTTCCTGATTATGATCATCCTGACTGTGATTTCCCTGGGTGTAATCTCTGTTTACGCTCCGGCTGCCACTAAGAAAAAGCCGATTCCGGTGCGCCTGATCAAACGTAAAAAGTACTACGCTATCATCGTTTCCCTGACCCTGTTCATTATCACCCTGATTATCAAAGAACCGTTTGCACAGTTCATCCAGCTGGGTATTATCATCGAAGCGATCACTCTGCTGCCGATCTTTTTCATCAAAGAGGATCTGAAATAAtactagtagcggccgctgcaggcggc</pre> | |||
==AgrC== | |||
<pre>atgaattcgcggccgcttctagagATGATTCTGATGTTCACCATTCCAGCAATTATTAGCGGTATTAAGTACAGCAAGCTGGATTATTTCTTCATCATCGTAATCTCTACCCTGTCCCTGTTCCTGTTCAAAATGTTCGATTCCGCGAGCCTGATTATCCTGACTTCCTTCATCATCATCATGTATTTCGTAAAAATCAAATGGTATAGCATCCTGCTGATCATGACCTCCCAGATTATCCTGTACTGTGCCAACTACATGTATATCGTTATCTACGCGTACATCACGAAAATCTCCGATTCTATCTTCGTTATCTTTCCGTCTTTCTTCGTGGTGTACGTTACGATCTCTATTCTGTTTTCCTATATTATCAATCGCGTTCTGAAAAAGATCTCTACTCCGTACCTGATTCTGAACAAAGGTTTTCTGATCGTCATCTCCACTATCCTGCTGCTGACCTTCTCTCTGTTCTTCTTTTACTCTCAGATCAACTCCGATGAGGCAAAAGTCATCCGTCAGTACAGCTTCATCTTTATCGGTATTACTATCTTCCTGAGCATTCTGACTTTCGTTATCAGCCAGTTCCTGCTGAAAGAAATGAAGTACAAGCGCAACCAAGAAGAAATCGAGACTTACTACGAGTACACCCTGAAGATTGAAGCGATCAACAACGAAATGCGTAAATTTCGTCACGACTACGTTAACATTCTGACCACCCTGAGCGAATACATCCGTGAAGACGACATGCCTGGCCTGCGTGATTATTTCAACAAAAACATTGTGCCGATGAAAGATAACCTGCAAATGAACGCTATTAAGCTGAACGGTATCGAAAACCTGAAAGTACGTGAGATCAAGGGTCTGATTACCGCTAAAATCCTGCGTGCGCAGGAAATGAACATCCCAATTAGCATCGAAATCCCAGACGAAGTTTCTAGCATTAACCTGAACATGATTGACCTGTCTCGTAGCATCGGCATCATCCTGGACAACGCTATCGAAGCTTCCACTGAAATCGACGACCCGATTATTCGCGTTGCTTTTATCGAGAGCGAAAACTCCGTGACCTTTATCGTGATGAACAAATGCGCGGACGATATCCCGCGTATTCACGAACTGTTCCAGGAATCTTTCAGCACCAAGGGTGAAGGTCGCGGCCTGGGCCTGTCCACCCTGAAAGAGATCGCGGATAACGCCGACAACGTTCTGCTGGACACTATTATCGAAAACGGTTTCTTCATTCAGAAAGTCGAAATCATCAATAACTAAtactagtagcggccgctgcaggcggc</pre> | |||
==AgrD== | |||
<pre>atgaattcgcggccgcttctagagATGAATACTCTGTTCAATCTGTTTTTCGATTTCATTACTGGCATCCTGAAGAACATCGGTAACATCGCAGCGTACAGCACCTGCGACTTCATCATGGATGAAGTAGAGGTCCCGAAAGAGCTGACCCAGCTGCATGAATAAtactagtagcggccgctgcaggcggc</pre> | |||
==pXyl Promoter== | ==pXyl Promoter== | ||
With BB sites: 163 | |||
Without: 115 | |||
<pre>atgaattcgcggccgcttctagagGAAAAACTAAAAAAAATATTGAAAATACTGATGAGGTTATTTAAGATTAAAATAAGTTAGTTTGTTTGGGCAACAAACTAATGTGCAACTTACTTACAATATGACATAAAATGCAtactagtagcggccgctgcagatc</pre> | <pre>atgaattcgcggccgcttctagagGAAAAACTAAAAAAAATATTGAAAATACTGATGAGGTTATTTAAGATTAAAATAAGTTAGTTTGTTTGGGCAACAAACTAATGTGCAACTTACTTACAATATGACATAAAATGCAtactagtagcggccgctgcagatc</pre> | ||
==pSpac Promoter== | ==pSpac Promoter== | ||
With BB sites: 155 | |||
Without: 107 | |||
<pre>atgaattcgcggccgcttctagagAGAACAACCTCTGCTAAAATTCCTGAAAAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGGAATTGTGAGCGGATAACAATTAAGCTTtactagtagcggccgctgcagata</pre> | <pre>atgaattcgcggccgcttctagagAGAACAACCTCTGCTAAAATTCCTGAAAAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGGAATTGTGAGCGGATAACAATTAAGCTTtactagtagcggccgctgcagata</pre> | ||
==pPac Promoter== | ==pPac Promoter== | ||
With BB sites: 125 | |||
Without: 75 | |||
<pre>atgaattcgcggccgcttctagagAAACGAGGTCATCATTTCCTTCCGAAAAAACGGTTGCATTTAAATCTTACATATGTAATACTTTCAAAGACTACAtactagtagcggccgctgcaggcggc</pre> | <pre>atgaattcgcggccgcttctagagAAACGAGGTCATCATTTCCTTCCGAAAAAACGGTTGCATTTAAATCTTACATATGTAATACTTTCAAAGACTACAtactagtagcggccgctgcaggcggc</pre> | ||
==pUpp Promoter== | ==pUpp Promoter== | ||
<pre> | With BB sites: 285 | ||
Without: 239 | |||
<pre>gaattcgcggccgcttctagagATTTTTAAAGTATGTATACAAATGATGAATAAATTTTGGCGATATAATGAAGGATACAGCTCCCATAATTGGTAAAGATACTAGATAGATTCATCGTAAAATCATGATTTTGCCAAATTTGCCCTTGAATATTAGTAGCGTTTTCTTTACAATCGTAAATAGTGTAAAAAAGCGTGCAAACGCATGAATATCATCTAAAGGAGAGATTCACATGGGAAAACTGTATGTATTTGATCCTCtactagtagcggccgctgcagat</pre> | |||
==consensus B. subtilis RBS== | ==consensus B. subtilis RBS== | ||
<pre> | With BB sites: 54 | ||
Without: 11 | |||
<pre>gaattcgcggccgcttctagagAAAGGAGG TGTtactagtagcggccgctgcag</pre> | |||
==weak B. subtilis RBS== | |||
With BB sites: 54 | |||
Without: 11 | |||
<pre>gaattcgcggccgcttctagagAGAGGTGG TGTtactagtagcggccgctgcag</pre> | |||
Latest revision as of 09:46, 19 September 2008
Lowercase letters are biobrick ends, uppercase are the actual pieces.
AgrA
atgaattcgcggccgcttctagagATGGAAATCGCACTGGCTACGGACAATCCATATGAGGTTCTGGAACAGGCTAAAAACATGAACGACATCGGCTGCTATTTCCTGGATATTCAGCTGAGCACCGACATCAACGGTATCAAACTGGGCTCTGAAATCCGTAAACATGATCCGGTTGGTAACATTATTTTCGTGACTTCTCATTCTGAGCTGACCTATCTGACGTTCGTATACAAGGTTGCAGCCATGGATTTCATCTTTAAAGACGATCCGGCAGAGCTGCGTACGCGCATCATCGACTGTCTGGAAACTGCGCACACGCGCCTGCAACTGCTGTCCAAAGATAACTCCGTGGAAACCATTGAACTGAAACGTGGCAGCAACAGCGTTTACGTTCAGTACGATGATATCATGTTCTTCGAAAGCTCCACTAAGTCCCACCGTCTGATCGCGCATCTGGATAATCGTCAGATCGAGTTCTATGGCAACCTGAAAGAGCTGAGCCAGCTGGACGATCGCTTCTTCCGTTGTCATAACTCCTTCGTTGTTAACCGTCACAACATCGAATCCATCGACTCCAAAGAGCGTATTGTATACTTCAAGAACAAAGAACACTGTTATGCGTCTGTACGCAATGTCAAGAAAATCTAAtactagtagcggccgctgcaggcggc
AgrB
atgaattcgcggccgcttctagagATGAACTATTTTGACAACAAGATCGACCAATTTGCTACTTACCTGCAAAAACGCAACAACCTGGACCACATCCAGTTTCTGCAAGTTCGTCTGGGCATGCAGGTTCTGGCTAAAAACATCGGCAAACTGATCGTTATGTACACCATCGCCTATATTCTGAACATCTTCCTGTTTACCCTGATCACCAACCTGACCTTCTATCTGATTCGCCGTCATGCCCACGGCGCTCATGCACCTTCTTCCTTTTGGTGTTATGTAGAGAGCATTATTCTGTTTATCCTGCTGCCGCTGGTAATCGTTAACTTCCACATCAATTTCCTGATTATGATCATCCTGACTGTGATTTCCCTGGGTGTAATCTCTGTTTACGCTCCGGCTGCCACTAAGAAAAAGCCGATTCCGGTGCGCCTGATCAAACGTAAAAAGTACTACGCTATCATCGTTTCCCTGACCCTGTTCATTATCACCCTGATTATCAAAGAACCGTTTGCACAGTTCATCCAGCTGGGTATTATCATCGAAGCGATCACTCTGCTGCCGATCTTTTTCATCAAAGAGGATCTGAAATAAtactagtagcggccgctgcaggcggc
AgrC
atgaattcgcggccgcttctagagATGATTCTGATGTTCACCATTCCAGCAATTATTAGCGGTATTAAGTACAGCAAGCTGGATTATTTCTTCATCATCGTAATCTCTACCCTGTCCCTGTTCCTGTTCAAAATGTTCGATTCCGCGAGCCTGATTATCCTGACTTCCTTCATCATCATCATGTATTTCGTAAAAATCAAATGGTATAGCATCCTGCTGATCATGACCTCCCAGATTATCCTGTACTGTGCCAACTACATGTATATCGTTATCTACGCGTACATCACGAAAATCTCCGATTCTATCTTCGTTATCTTTCCGTCTTTCTTCGTGGTGTACGTTACGATCTCTATTCTGTTTTCCTATATTATCAATCGCGTTCTGAAAAAGATCTCTACTCCGTACCTGATTCTGAACAAAGGTTTTCTGATCGTCATCTCCACTATCCTGCTGCTGACCTTCTCTCTGTTCTTCTTTTACTCTCAGATCAACTCCGATGAGGCAAAAGTCATCCGTCAGTACAGCTTCATCTTTATCGGTATTACTATCTTCCTGAGCATTCTGACTTTCGTTATCAGCCAGTTCCTGCTGAAAGAAATGAAGTACAAGCGCAACCAAGAAGAAATCGAGACTTACTACGAGTACACCCTGAAGATTGAAGCGATCAACAACGAAATGCGTAAATTTCGTCACGACTACGTTAACATTCTGACCACCCTGAGCGAATACATCCGTGAAGACGACATGCCTGGCCTGCGTGATTATTTCAACAAAAACATTGTGCCGATGAAAGATAACCTGCAAATGAACGCTATTAAGCTGAACGGTATCGAAAACCTGAAAGTACGTGAGATCAAGGGTCTGATTACCGCTAAAATCCTGCGTGCGCAGGAAATGAACATCCCAATTAGCATCGAAATCCCAGACGAAGTTTCTAGCATTAACCTGAACATGATTGACCTGTCTCGTAGCATCGGCATCATCCTGGACAACGCTATCGAAGCTTCCACTGAAATCGACGACCCGATTATTCGCGTTGCTTTTATCGAGAGCGAAAACTCCGTGACCTTTATCGTGATGAACAAATGCGCGGACGATATCCCGCGTATTCACGAACTGTTCCAGGAATCTTTCAGCACCAAGGGTGAAGGTCGCGGCCTGGGCCTGTCCACCCTGAAAGAGATCGCGGATAACGCCGACAACGTTCTGCTGGACACTATTATCGAAAACGGTTTCTTCATTCAGAAAGTCGAAATCATCAATAACTAAtactagtagcggccgctgcaggcggc
AgrD
atgaattcgcggccgcttctagagATGAATACTCTGTTCAATCTGTTTTTCGATTTCATTACTGGCATCCTGAAGAACATCGGTAACATCGCAGCGTACAGCACCTGCGACTTCATCATGGATGAAGTAGAGGTCCCGAAAGAGCTGACCCAGCTGCATGAATAAtactagtagcggccgctgcaggcggc
pXyl Promoter
With BB sites: 163 Without: 115
atgaattcgcggccgcttctagagGAAAAACTAAAAAAAATATTGAAAATACTGATGAGGTTATTTAAGATTAAAATAAGTTAGTTTGTTTGGGCAACAAACTAATGTGCAACTTACTTACAATATGACATAAAATGCAtactagtagcggccgctgcagatc
pSpac Promoter
With BB sites: 155 Without: 107
atgaattcgcggccgcttctagagAGAACAACCTCTGCTAAAATTCCTGAAAAATTTTGCAAAAAGTTGTTGACTTTATCTACAAGGTGTGGCATAATGTGTGGAATTGTGAGCGGATAACAATTAAGCTTtactagtagcggccgctgcagata
pPac Promoter
With BB sites: 125 Without: 75
atgaattcgcggccgcttctagagAAACGAGGTCATCATTTCCTTCCGAAAAAACGGTTGCATTTAAATCTTACATATGTAATACTTTCAAAGACTACAtactagtagcggccgctgcaggcggc
pUpp Promoter
With BB sites: 285 Without: 239
gaattcgcggccgcttctagagATTTTTAAAGTATGTATACAAATGATGAATAAATTTTGGCGATATAATGAAGGATACAGCTCCCATAATTGGTAAAGATACTAGATAGATTCATCGTAAAATCATGATTTTGCCAAATTTGCCCTTGAATATTAGTAGCGTTTTCTTTACAATCGTAAATAGTGTAAAAAAGCGTGCAAACGCATGAATATCATCTAAAGGAGAGATTCACATGGGAAAACTGTATGTATTTGATCCTCtactagtagcggccgctgcagat
consensus B. subtilis RBS
With BB sites: 54 Without: 11
gaattcgcggccgcttctagagAAAGGAGG TGTtactagtagcggccgctgcag
weak B. subtilis RBS
With BB sites: 54 Without: 11
gaattcgcggccgcttctagagAGAGGTGG TGTtactagtagcggccgctgcag
