IGEM:Cambridge/2008/Turing Pattern Formation/Experiments

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Finding and Biobricking proper Ribosomal Binding Sites

• Solving Biobrick RBS issues

Testing Sender and Reciever

• We can test one, then use to test the other, or we can test them in parallel

Testing AgrC Reciever

• Use purified AIP
  • Synthesized
  • Sourced
    • Possibly from Tom Muir at Rochester
  • S. aureus purified supernatant (requires Pathology department personnel)
    • Guangyong Ji at Catholic University has a protocol for purification

Testing AgrA Transcription Factor and P2 promoter

• Can we get an 'always on' mutant my modifying some residues? (Jim's Idea)

Testing AgrB Exporter

• Membrane Localization Test
  • Can use membrane fractionation
  • Can use antibody tags with EM and gold

Testing AgrD Prepeptide/AIP

• Mass Spec Assay
  • Daniel Goodman 11:47, 31 July 2008 (UTC):Speaking to Kathryn Lilley in Proteomics
• S. aureus Reporter Strain
  • Daniel Goodman 11:47, 31 July 2008 (UTC):We cannot use staph ourselves, checking to see if we can get someone in Pathology department to test for us