IGEM:Cambridge/2008/Turing Pattern Formation/Primers: Difference between revisions
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(New page: ====Bacillus RBS Primers==== We made primer sets for 2 ribosomal binding sites in Bacillus of differing binding strengths. We expect B._sub_RBSs to bind very strongly because it is the co...) |
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====Bacillus RBS Primers==== | ====Bacillus RBS Primers==== | ||
We made primer sets for 2 ribosomal binding sites in Bacillus of differing binding strengths. We expect B._sub_RBSs to bind very strongly because it is the consensus sequence for RBS in B. subtilis. | We made primer sets for 2 ribosomal binding sites in Bacillus of differing binding strengths. We expect B._sub_RBSs to bind very strongly because it is the consensus sequence for RBS in B. subtilis. | ||
<code> | |||
B. subtilis consensus RBS - http://www.ncbi.nlm.nih.gov/pubmed/10446248 | B. subtilis consensus RBS - http://www.ncbi.nlm.nih.gov/pubmed/10446248 | ||
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B._sub_RBSs_R | B._sub_RBSs_R | ||
*5' CTGCAGCGGCCGCTACTAGTAACA CCTCCTTT CTCT 3' | *5' CTGCAGCGGCCGCTACTAGTAACA CCTCCTTT CTCT 3'</code> | ||
RBS modified from ECE112 SpoVG RBS - http://www.bgsc.org/Catalogs/Catpart4.pdf | <code>RBS modified from ECE112 SpoVG RBS - http://www.bgsc.org/Catalogs/Catpart4.pdf | ||
B._sub_RBSw_F | B._sub_RBSw_F | ||
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B._sub_RBSw_R | B._sub_RBSw_R | ||
*5' CTGCAGCGGCCGCTACTAGTAACA CCACCTCT CTCT 3' | *5' CTGCAGCGGCCGCTACTAGTAACA CCACCTCT CTCT 3'</code> | ||
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=====agr senders===== | =====agr senders===== | ||
Agr_B_F | <code>Agr_B_F | ||
*5' GTTTCTT C GAATTC GCGGCCGC T TCTAG ATGaactattttgacaacaa 3' | *5' GTTTCTT C GAATTC GCGGCCGC T TCTAG ATGaactattttgacaacaa 3' | ||
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Agr_D_R | Agr_D_R | ||
*5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA ttcatgcagctgggtcagct 3' | *5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA ttcatgcagctgggtcagct 3'</code> | ||
=====agr receivers===== | =====agr receivers===== | ||
Agr_C_F | <code>Agr_C_F | ||
*5' GTTTCTT C GAATTC GCGGCCGC T TCTAG atgattctgatgttcaccat 3' | *5' GTTTCTT C GAATTC GCGGCCGC T TCTAG atgattctgatgttcaccat 3' | ||
| Line 52: | Line 51: | ||
Agr_A_R | Agr_A_R | ||
*5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA gattttcttgacattgcgta 3' | *5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA gattttcttgacattgcgta 3' | ||
</code> | |||
Revision as of 09:36, 5 August 2008
Bacillus RBS Primers
We made primer sets for 2 ribosomal binding sites in Bacillus of differing binding strengths. We expect B._sub_RBSs to bind very strongly because it is the consensus sequence for RBS in B. subtilis.
B. subtilis consensus RBS - http://www.ncbi.nlm.nih.gov/pubmed/10446248
B._sub_RBSs_F
- 5' GAATTCGCGGCCGCTTCTAGAG AAAGGAGG TGTTA 3'
B._sub_RBSs_R
5' CTGCAGCGGCCGCTACTAGTAACA CCTCCTTT CTCT 3'
RBS modified from ECE112 SpoVG RBS - http://www.bgsc.org/Catalogs/Catpart4.pdf
B._sub_RBSw_F
- 5' GAATTCGCGGCCGCTTCTAGAG AGAGGTGG TGTTA 3'
B._sub_RBSw_R
5' CTGCAGCGGCCGCTACTAGTAACA CCACCTCT CTCT 3'
Agr Primers
- These primers are to extract each part of the Agr operon so that a Bacillus RBS can replace the E. coli RBS currently attached to the genes.
agr senders
Agr_B_F
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAG ATGaactattttgacaacaa 3'
Agr_B_R
- 5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA tttcagatcctctttgatg 3'
Agr_D_F
- 5' CTT C GAATTC GCGGCCGC T TCTAG atgaatactctgttcaatctgtttt 3'
Agr_D_R
5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA ttcatgcagctgggtcagct 3'
agr receivers
Agr_C_F
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAG atgattctgatgttcaccat 3'
Agr_C_R
- 5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA gttattgatgatttcgactt 3'
Agr_A_F
- 5' GTTTCTT C GAATTC GCGGCCGC T TCTAG atggaaatcgcactggcta 3'
Agr_A_R
- 5' TT C CTGCAG CGGCCGC T ACTAGT A TTATTA gattttcttgacattgcgta 3'
