IGEM:Cambridge/2008/Turing Pattern Formation/Vectors: Difference between revisions
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All these vectors were kindly provided by Dr. Dan Ziegler, Director of the Bacillus Genetic Stock Center at Ohio State University. | All these vectors were kindly provided by Dr. Dan Ziegler, Director of the Bacillus Genetic Stock Center at Ohio State University. | ||
*'''[[User:Daniel Goodman|Daniel Goodman]] 17:01, 31 July 2008 (UTC)''':I just put this table together, but I have yet to add the plasmid maps to these. For now, to see the maps, go to [http://bgsc.org BGSC homepage] and find them in the catalog. They are also in the black Turing Group binder under "ordered vectors and strains". | |||
= Integration Vectors = | = Integration Vectors = |
Revision as of 10:01, 31 July 2008
All these vectors were kindly provided by Dr. Dan Ziegler, Director of the Bacillus Genetic Stock Center at Ohio State University.
- Daniel Goodman 17:01, 31 July 2008 (UTC):I just put this table together, but I have yet to add the plasmid maps to these. For now, to see the maps, go to BGSC homepage and find them in the catalog. They are also in the black Turing Group binder under "ordered vectors and strains".
Integration Vectors
These vectors integrate into the chromosome and do not have a replication origin in Bacillus. They either integrate cassettes that require double crossovers and two homologous regions, or whole-plasmid insertions that only require one crossover and one corresponding region of homology.
Ectopic Integration
The integration site of these is pre-determined
BGSC Accession | Vector Name | Features | Integration Locus | Vector Map | Vector Sequence |
---|---|---|---|---|---|
ECE112 | pDG1661 |
|
amyE | map | U46196 |
ECE171 | pPyr-Kan |
|
pyrD | map | AY464559 |
ECE172 | pGlt-Cm |
|
gltA | map | AY464560 |
ECE176 | pBCJ164 |
|
rspD | map | BGSC sequence |
ECE153 | pSG1154 |
|
amyE | map | not available |
ECE162 | pSG1193 |
|
amyE | map | not available |
Insert Integration
The user inserts a homologous piece of the Bacillus chromosome and the vector integrates there.
BGSC Accession | Vector Name | Features | Vector Map | Vector Sequence |
---|---|---|---|---|
ECE149 | pMUTIN-GFP+ |
|
map | From Bayreuth |
ECE150 | pMUTIN-CFP |
|
map | From Bayreuth |
ECE151 | pMUTIN-YFP |
|
map | From Bayreuth |
ECE147 | pMUTIN-cMyc |
|
map | From Bayreuth |
Shuttle Vectors
BGSC Accession | Vector Name | Features | Vector Map | Vector Sequence |
---|---|---|---|---|
ECE165 | pAD123 |
|
map | BGSC sequence |
ECE166 | pAD43-25 |
|
map | BGSC sequence |
ECE188 | pHCMC02 |
|
map | From Bayreuth |
ECE189 | pHCMC04 |
|
map | From Bayreuth |
ECE190 | pHCMC05 |
|
map | From Bayreuth |