IGEM:China workshop/Participants

PKU Team

THU Team Introduction As the school motto “Self-discipline and Social Commitment” reflects, Tsinghua University iGEM 2007 team is focusing on endeavor and creativity as well as teamwork and responsibility in the process of cooperating with the others to accomplish a goal. The team consists of two supervisors, three advisors, eight members and the desire to explore the world of synthetic biology. Team Composition Supervisors Prof. Chen Guoqiang Dr. Xu Feng Advisors Liu Zhe: PhD student in Laboratory of Structural Biology, Tsinghua University Shi Zhenyu: Senior undergraduate student majoring in fundamental science (Chemistry and Biology) Song Xindong: PhD student in Medical Systems Biology Research Center Team Members Chen Chen: Senior undergraduate majoring in Biology Dong Peng: PhD student in Medical Systems Biology Research Center Jiang Ming: Junior undergraduate majoring in Electronic Engineering Liu Yexing: Junior undergraduate majoring in Biology Li Keyu: Junior undergraduate majoring in Biology Yu Zhou: unior undergraduate majoring in Biology Zhao Xinyu: enior undergraduate majoring in Biology Zhou Lijun: Junior undergraduate majoring in Biology Lab Prof. Chen Guoqiang kindly provides us working space and reagents for experiments in his lab of Microbiology, Tsinghua University Preliminary Proposals E. coli Communicating Chain Construction How much can we disturb or control the communication between microorganisms in tremendous amount? Perhaps a chain carrying signals that we want to bruit is possible to be constructed in the society of E. coli. Then we can gain a closer insight into those tiny lives. E. coli Oscillator According to former work in the realm of Oscillator, we are inspired to design special gene sequence that can endow E. coli periodical reaction in certain forms to certain signals, which can be derived from other cells or be opposed artificially. Contact Us The following is the contact of the Tsinghua University iGEM 2007 team. Please feel free to contact us, we would be more than happy to communicate with you. Your advice and suggestions are welcome and would be highly appreciated. Address: Tsinghua University IGEM Team C/O Liu Yexing, Undergraduate Student in Biology, Tsinghua University, Department of Biological Science and Technology, Haidian District, Beijing, 100084, China Email:yexing.liu@gmail.com Tel:+86-010-515-34104 Mobile:+86-138-1177-1678

TJU Team Team one Team one Introduction With the great passion, innovative ideas and team work spirit, students from Tianjin University Team 1 are actively participating in this competition. We explain the iGEM competition as an international platform to show our great passion, energetic, most creative. Sound financial and technological strength is what we rely on to do a good job in this competition. We all have great power and are filled with confidence. Just to say “Go on, we will succeed”. Team members: Supervisors: Prof. Yuan Yingjin Dr. Cheng Jingsheng Dr. Song Kai Advisors Li Bingzhi: PhD student majoring in Biochemical Engineering Wang Honglei: Graduate student majoring in Chemical Engineering Hu Bo: Graduate student majoring in Biochemical Engineering Wang Xing: Graduate student majoring in pharmaceutical Engineering Zhou Jian: Graduate student majoring in pharmaceutical Engineering Shi Lei: Graduate student majoring in Biochemical Engineering Team Members Sun jingjing: Senior undergraduate majoring in Bioengineering Li Xiaoqian: Senior undergraduate majoring in Bioengineering Zhu Manli: Senior undergraduate majoring in Bioengineering Zou ruiyang: Senior undergraduate majoring in Molecular Science and Technology Qiao Kangjian: Senior undergraduate majoring in Chemical Engineering Chen Zheng: Senior undergraduate majoring in Chemical Engineering Lab and Funding Prof. YJ Yuan kindly provides experiment environment and most of the financial support. Also our team gets the financial support from the National Natural Science Foundation of China and the Tianjin University. Primary proposals: Synthetic Biology related technology Logic gate(AND,OR,NOT,NAND,NOR,XOR):We combine some of them to construct several devices, such as DOUBLE CONTROL SWITCH, NUMERUS COMPARATOR, INCONSISTENT CIRCUIT, SR FLIP FLOP, ENCODER, CLOCK, much of them come from the electrocircuit consideration. Simulate reversible reaction: We try to simulate the reversible reaction with considering the impact of catalyzer, temperature, concentration and pressure. To use two fluorescence reporters, we can monitor the reaction direction by the colour varying. System controlling lytic/ lysogenic states: By cell-to-cell communication, we try to construct a system that a signal from a cell can control the lytic/ lysogenic states of cell infected by phage and using different reporter according to the different state. As we all know, time delay is very common in biology system. Is there some method that we can take use of this time delay? So we can use it to all the situations change is needed. Because biological system is unpredictable and couldn’t control strictly as electrocircuit, there are still many problems we must face. We proceed with all the ideas but focus on the most feasible. Other inspiration Besides the logic application in our biological machine, we also hope to find other inspiration in energy and medicine issues. Surely, we get closer and closer to the final project. We are looking forward to your attention to our team Team two Introduction We come from Tianjin University, one of the most famous universities in China. Our team is composed of fifteen creative young people with enormous passion for synthetic biology. The solid foundation of professional knowledge provides us firm confidence, and the enthusiastic research spirit gives us the courage to challenge the mystery of science. We believe that everything can be possible with our endeavor and wisdom. Members Graduates Binbin Han PHD of biochemical engineering Shuobo Shi PHD of biochemical engineering Hui Wang PHD of biochemical engineering Tong Hao Master of biochemical engineering Zongbao Zheng Master of biochemical engineering Yanfeng Xue Master of biochemical engineering Hao Ma Master of biochemical engineering Jie Zhang Master of Zymolysis engineering Undergraduates Xiaowen Hou major in biological engineering Liwei Chen major in biological engineering Hong Zhang major in biological engineering Liujie Huo major in biological engineering Bo Wang major in biological engineering Yifan Yuan major in biological engineering Shunhua Yang major in biological engineering Faculty Advisors Prof. X.M. Zhao Prof. Q.Z. Wang Lab and funding Prof. X.M. Zhao is kind enough to provide ‘wet’ experiments environment and ‘dry’ experiment equipment for us. Project 1 ene circuit The aim of our project is to simulate electrocircuit in series and parallel connection by gene elements. Series circuit and parallel circuit are the simplest circuits in physics. There are three eletments in it: switch, power and resistance. What we should do is to construct three kinds of bacterials functioning as the three elements in electrocircuit, seperately: one for power, the other two for resistances(R1 and R2). Light is used as a switch. The gene circuit is connected when the bacterials are supposed to light so that the flurescence proteins with different colors in the three kind of bacterials are all expressed, while the circuit is cut when we turn the light off. In series circuit, If we cut any of the resistances or power, it will result in the disconnection of the whole circuit, while in parallel circuit, the lack of one resistance will not influence the other one. Three different kinds of HSL are used as the cell to cell signals. Project 2 Bacterial neon light In this project ,we use gene circle to create a bacterial neon light with three different colors. the first repressor protein, LacI from E. coli, inhibits the transcription of the second repressor gene, tetR from the tetracycline-resistance transposon Tn10, whose protein product in turn inhibits the expression of a third gene, cI from λ phage. Finally, CI inhibits lacI expression, completing the cycle. The three repressors are connected with red, green, and yellow luc genes (a kind of luminescent gene) seperately. With the oscillation of the gene circle, the three colors of light will show up circularly, just as a neon light. Contact The following is the contact of the iGEM TJU 2007 team. Your advices and suggestions are welcome and highly appreciated. Address:Room 310, Chemical Engineering Department, Tianjin University . Tianjin .P.R.China (300072) Email:haotong2001@sina.com Tel:+86-022-27892075 Team three team three

USTC Team Introduction We come from USTC, one of the most famous universities in China. We are the combination of the exact tradition of USTC and the exact vitality of the young. Rigorous daily schoolwork has provided us with a solid foundation of basic knowledge, and an innovative spirit enables us to stand on the frontier of the modern science. Our belief is'Never to say failur'. Member Graduates Zhan Jian : PHD candidate in Key Laboratory of Structural Biology Ding Bo: PHD candidate in Key Laboratory of Structural Biology Ma Rui: PHD student in Laboratory of Nonlinear Chemistry Ma Xiaoyu: PHD student in National Laboratory for Physical Sciences at Microscale Undergraduates Su Xiaofeng: major in molecular and cellular Biology Liu Ziqing: major in molecular and cellular Biology Faculty Advisors Prof. H.Y. Liu Prof. J.R. Wu Prof. Z.H. Hou Lab and funding Prof. J.R. Wu is kind enough to provide ‘wet’ experiments environment. Our team is sponsored by the National Science Funding and the CYL committee of USTC. Proposal Despite the exciting advancement in recent years, phage contamination is still one of the headaches that nowadays the food and fermentation industries mainly confront. Some strains with genotype like TonA is resistant to T1-like bacteriophages, but the productive performance of these strains is low because of the slow growth rates. Therefore, the producer is facing a dilemma: how can we deal with the strains lacking phage resistance, but with high productivity, and strains with certain kind of phage resistance but the productivity of which is rather low. To help those unfortunate guys, we aim at developing an inexpensive system to offer the industries a new kind of strain that can grow fast and without phage infection. There are two different strategies for the active defense: One is the ‘Signaler Strategy’, that is, let a cell infected with bacteriophages send a signal telling other cells that the enemy is coming. In this way the others will switch on the expression of TonA , making themselves resistant to the enemy. There are two key points for this strategy. Firstly, what can be used as such a signal? It should be rare in the environment in most occasions, and its number ought to increase fast after the infection. Secondly, how to switch on the expression of TonA? The response should be as quick as possible, or the enemy will invade in without any resistance! The other strategy is the ‘Sacrifice Strategy’, that is, when the phage begins to reproduce itself, the apoptosis system of the host will be switched on. The host commits suicide to avoid the propagation of the phages, and without influencing the others. Also, there is a key point for this strategy, that is, how to make the reaction as quick as possible .And another one is how to keep the apoptosis off under normal conditions. Both of the strategies might be applied to reinforce the self-defense ability of cells. Contact The following is the contact of the iGEM USTC 2007 team. Your advice and suggestions are welcome and highly appreciated. Address:Room 456, Life Sciences Building, University of Science and Technology of China Hefei, Anhui, P.R.China (230027) Email:zhanjian@ustc.edu Tel:+86-551-3603754-1 Mobile:+86-13856942160