IGEM:Groningen/Notebook/iGEM 2011/2011/05/31: Difference between revisions
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Joyce Mulder (talk | contribs) (Autocreate 2011/05/31 Entry for IGEM:Groningen/Notebook/iGEM_2011) |
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== | ==31-5-11== | ||
<br>Transformation of overnight ligation | |||
<br>Add to 40μl competent cells 10μl ligation mixture | |||
<br>Incubate for 30 min on ice | |||
<br>Heat shock: 45s at 42 degrees | |||
<br>Incubate cells on ice for 2 min | |||
<br>Add 1ml LB medium+ 25mM glucose | |||
<br>Incubate cells for 1h/1.5h at 37 degrees | |||
<br>Spin the cells down, resuspend them in 100μl LB medium and plate 90μl and 10μl out on the plates | |||
<br>Put the plates in the stove at 37 degrees overnight | |||
<br> | |||
<br>Doing plasmid prep of overnight cultures | |||
<br>Working on Human practices | |||
<br> | |||
Revision as of 01:27, 21 September 2011
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